Creative Biogene Develops Knockout Cell Line Generation as A New Method for Genome Editing

September 23 18:52 2019

New York, Sep 23rd, 2019 – Creative Biogene, the world’s leading biotechnology products and services provider, recently announced the new release of Stable Knockout Cell Line Generation service. CRISPR/Cas9 system, as a newly developed method for genome editing, which has been widely used in current biology, functional genomic screening, cell-based human genetic disease modeling, epigenetic genomics research, and visualization of cellular processes. Besides, the simplicity and efficiency of the CRISPR/Cas9 system make it the preferred genomic knockout method for traditional ZFN and TALEN systems.

 

CRISPR/Cas9 has been widely utilized to facilitate functional knockout of protein-coding genes. However, a single genetic editing site may not be sufficient to disrupt the structure and function of the protein. Creative Biogene is experienced in producing fragment-deleting cell lines using any mammalian cell line and targeting sequences, the use of two sgRNAs that target upstream and downstream direct sequences is a viable method for fully target functional knockout of target proteins.

 

In addition, multiplexing with CRISPR/Cas9 can accelerate genomic engineering in biopharmaceutical research and drug discovery. Creative Biogene is capable to provide additional conversions and selection rounds until each target gene is eliminated. Mammalian cells exposed to two or three gRNAs in turn result in cell lines with double or triple knockouts, all of which are obtained by DNA mutations in the target gene and have the expected knockout Phenotype. It can be applied to target screening of important specific pathways and specific biological processes and used as a screening tool for drug development.

 

The advantage of CRISPR/Cas9 over previous forms of gene editing is that it is simpler to implement and more efficient in performing biallelic modifications. Creative Biogene can provide a complete CRISPR/Cas9-based single gene knockout service using any mammalian cell line and target any gene. Our service ranges from host cell characterization, gRNA design and KO vector construction to transfection, single cell screening and validation.

 

Except for professional gene knock out cell line generation services, Creative Biogene also offers a range of knockout cell lines developed by the CRISPR/Cas9 system, which can provide a convenient way to study gene function and design disease models. Our knockout stable cell lines have been rigorously tested and validated. Target genes can be stably inherited for more than 25 generations and avoid mycoplasma contamination.

 

“For the result deliberation, we can offer reports on the Cas9-sgRNA vectors used for KO and the sgRNA sequence, and Sanger sequencing data showing that there are frameshift mutations in each clone.” said Macia Brady, the marketing director of Creative Biogene, she also claimed, “our one-stop service is provided from design of gRNAs, cell transfection, preparing of single clones to sequencing analysis, and the in-house experts can guarantee the effective results for your research.”

 

About Creative Biogene

Creative Biogene, as a top-of-its-kind company who holds a leading position and is committed to accelerating the development of human science through biotechnology, offering innovative technologies, products, unique tools and services for research discoveries and product development. With more than 10 years of experience and in-house experts, Creative Biogene has become a well-recognized industry leader to support researchers worldwide.

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Company Name: Creative Biogene
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Phone: 6316197922
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Website: https://www.creative-biogene.com